farFRET: Extending the Range in Single-Molecule FRET Experiments beyond 10 nm
A recent report in Nano Letters (1), describes the development of farFRET and demonstrates that farFRET can be readily employed to quantify FRET efficiencies and conformational dynamics using DNA rulers, RecA-filament formation on single-stranded DNA and Holliday junction dynamics. farFRET allows quantitative measurements of large biomolecular complexes and nanostructures, thus, bridging the remaining gap to superresolution microscopy.
Michael Schlierf is a group leader (assistant professor) at TU Dresden in B CUBE – Center for Molecular Bioengineering since fall 2010. Before that, he worked as a DFG Postdoctoral fellow and CPLC associate with Taekjip Ha at the University of Illinois (Urbana-Champaign) from 2008 to 2010. For more information, see www.tu-dresden.de/bcube/schlierf
(1) Krainer, G., Hartmann, A., & Schlierf, M. (2015). farFRET: Extending the Range in Single-Molecule FRET Experiments beyond 10 nm. Nano Letters. http://doi.org/10.1021/acs.nanolett.5b01878