3rd Midwest Single Molecule Workshop 2014
University of Illinois at Urbana-Champaign
August 4-5, 2014
Alice Campbell Alumni Center

Schedule

View the detailed program of the workshop.

Monday, August 4, 2014
8:00 a.m. - 8:45 a.m. Registration & coffee
8:45 a.m. - 9:00 a.m. Introduction and welcome
9:00 a.m. - 10:00 a.m. Keynote lecture: Stephen Kowalczykowski, UC Davis
10:00 a.m. - 10:20 a.m. Coffee break
Session I: Single-molecule interactions
10:20 a.m. - 10:40 a.m. Talk 1: Sanjeevi Sivasankar, Iowa State University
"Conformational switching in single prion proteins promotes oligomerization"
10:40 a.m. - 11:00 a.m. Talk 2: Charles Schroeder, University of Illinois
"Direct observation of TALE protein search dynamics along DNA"
11:00 a.m. - 11:20 a.m. Talk 3: Yi Luo, The Ohio State University
"Nucleosomes Accelerate Transcription Factor Dissociation"
11:20 a.m. - 11:40 a.m. Talk 4: Sujay Ray, Kent State University
"G-quadruplex formation in telomeres enhances POT1/TPP1 protection against RPA binding"
11:40 a.m. - 12:00 p.m. Talk 5: Krishna Sigdel, University of Missouri
"Mechanical insight into lipid-protein interactions using bee venom"
12:00 p.m. - 2:30 p.m. Lunch and poster session I
Session II: Molecular machines
2:30 p.m. - 2:50 p.m. Talk 6: Peter Cornish, University of Missouri
"Structured mRNA induces the ribosome into a hyper-rotated state"
2:50 p.m. - 3:10 p.m. Talk 7: Julia Widom, University of Michigan
"Dissecting the Functions of RNA Helicases in Splicing by Single-Molecule FRET"
3:10 p.m. - 3:30 p.m. Talk 8: Maria Spies, University of Iowa
"Single-molecule studies of FeS-containing DNA helicases: kinetics, conformational dynamics and molecular mechanisms"
3:30 p.m. - 3:50 p.m. Coffee break
3:50 p.m. - 4:10 p.m. Talk 9: Eric Tomko, Washington University in St. Louis
"ATP- and NTP-dependent promoter opening by the yeast RNAP II Pre-initiation complex"
4:10 p.m. - 4:30 p.m. Talk 10: Alfonso Brenlla, Wayne State University
"Mechanism of aromatic carcinogen bypass by the Y-family polymerase Dpo4"
4:30 p.m. - 6:30 p.m. Poster session II
7:00 p.m. Reception
Tuesday, August 5, 2014
8:00 a.m. - 8:50 a.m. Refreshments
8:50 a.m. - 9:00 a.m. Introduction & announcements
Session III: Single molecules in live cells
9:00 a.m. - 9:20 a.m. Talk 11: Kenneth Ritchie, Purdue University
"Mobility of TonB and FepA in the membranes of E. coli"
9:20 a.m. - 9:40 a.m. Talk 12: Wenting Li, University of Wisconsin-Madison
"Single Molecule Study of RelA during the stringent response in live E. coli cells"
9:40 a.m. - 10:00 a.m. Talk 13: Rudra Kafle, University of Michigan
"Dynamics of Chromosomal DNA in Escherichia coli"
10:00 a.m. - 10:20 a.m. Coffee break
10:20 a.m. - 10:40 a.m. Talk 14: Taekjip Ha, University of Illinois
"Single Molecules and Cellular Mechanics"
10:40 a.m. - 11:00 a.m. Talk 15: Yan Mei Wang, Washington University in St. Louis
"Single-molecule investigation of intraflagellar transport mechanisms"
11:00 a.m. - 11:20 a.m. Talk 16: Qiong Yang, University of Michigan
"From molecules to development: revealing simple rules of biological clocks"
11:20 a.m. - 11:50 a.m. Business meeting
11:50 a.m. - 12:00 p.m. Poster awards
12:00 p.m. - 1:00 p.m. Lunch
Session IV: New methods
1:00 p.m. - 1:20 p.m. Talk 17: Wei Cheng, University of Michigan
"Optical Trapping and Multi-parameter Analysis of Single HIV-1 in Culture Media Reveal the Positive Cooperativity of Envelope Spikes in Mediating Viral Infection"
1:20 p.m. - 1:40 p.m. Talk 18: Richelle Teeling-Smith, The Ohio State University
"Exploring Dynamics with Single Molecule Electron Paramagnetic Resonance"
1:40 p.m. - 2:00 p.m. Talk 19: Randall Goldsmith, University of Wisconsin-Madison
"What to do when the (fluorescent) lights go out: toward single molecule spectroscopy with optical microresonators"
2:00 p.m. - 2:20 p.m. Talk 20: Margaret Rodgers, University of Wisconsin-Madison
"A dual-functioning genetic tag for simultaneous isolation and observation of single fluorescent complexes from whole cell extract"
2:20 p.m. - 2:40 p.m. Talk 21: Aleksei Aksimentiev, University of Illinois
"Probing DNA-protein association through atomic and coarse-grained simulations"
2:40 p.m. - 3:00 p.m. Talk 22: Michael Hudoba, The Ohio State University
"Design of Force-Sensitive DNA Origami Components"
3:00 p.m. Closing remarks & departure