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CPLC investigating how protein folding differs in different tissue types of living zebrafish

3/15/2019 11:18:18 AM CPLC Staff

Quantifying protein dynamics and stability in a living organism

As an integral part of modern cell biology, fluorescence microscopy enables quantification of the stability and dynamics of fluorescence-labeled biomolecules inside cultured cells. However, obtaining time-resolved data from individual cells within a live vertebrate organism remains challenging. Here we demonstrate a customized pipeline that integrates meganuclease-mediated mosaic transformation with fluorescence-detected temperaturejump microscopy to probe dynamics and stability of endogenously expressed proteins in different tissues of living multicellular organisms.

 

2/7/2014 Christopher Tepedino (Smile Politely)

He starts with a lamp. When he turns it on, a bright square light permeates the room. It?s an invention made through his company Eden Park Illumination. The bright square light is powered by plasma. And that?s the topic of his lecture. The auditorium is packed. They?ve come to see a man skilled at his craft. Gary Eden is his name, and he?s a professor at the University of Illinois, Champaign-Urbana, in the field of engineering. He studies optics, lasers, and plasma, and the last of which is the focus of his lecture?what microplasma is and how it can help improve lives.