CPLC Visitor's Program

Detailed information on the visitor's program will be available in the future.

Signe Mathiasen and Sune Klamer Jørgensen, PhD students from the University of Copenhagen Signe and Sune are visiting the Ha lab from October 29, 2012 to January 14, 2013, to learn smFRET methods. While visiting they will study in vitro dimerization of G-protein coupled receptors reconstituted in liposomes at the single-molecule level. The project results from a collaboration between Professors Dimitrios Stamou and Soren Rasmussen (University of Copenhagen) and Professor Taekjip Ha (UIUC). For more information, please visit: http://nano.ku.dk/english/research/Nanobio/stamou_group/.

Dr. Keren Lasker, a postdoc with Dr. Lucy Shapiro at Stanford University, will be visiting the Luthey-Schulten lab in Summer 2012 to learn how to use the Lattice Microbe software for stochastic cell simulations on Caulobacter crescentus.

Rachid Rezgui, Postdoctoral Researcher, Laboratory for Optics and Biosciences, École Polytechnique  

Dr. Rezgui visited the Center in the Selvin Lab from April 1 to May 25, 2012. He started to use FIONA in order to get nanometer accuracy and to improve the lifetime of organic fluorophores via deoxygenation techniques. He worked also with SHREC for colocalization between single NucS enzymes and DNA molecules.

Katie Wilson, 3rd-Year Graduate Student in the Dave Fernig Research Lab at the Institute Integrative Biology, University of Liverpool, England visited the Selvin Lab generating small quantum dots—6. 2 nm, instead of the usual 21 nm diameter.

Vishal Kottadiel, Graduate Student, Rao Lab, Department of Biology, The Catholic University of America

Vishal visited the Center from May to December of 2009 and again from March 2011 to February 2012, working in the Chemla lab. He used high-resolution dual-trap optical tweezers to investigate the ATP-coupled dynamics of the packaging of the bacteriophage T4 DNA packaging motor. This work was presented at the Phage/Virus Assembly conference at Port Aransas in October 2011. He is also looking at co-ordination between the packaging motor subunits using fluorescent-labeled proteins and using the fleezers (CPLC Station 1) in order to decipher the packaging mechanism.

For more information, please visit:  http://biology.cua.edu/faculty/rao.cfm

Kuang Shen, Graduate student, Shu-ou Shan lab, California Institute of Technology

Kuang visited Ha lab from January 2012 to April 2012 to learn single molecule TIRF microscopy and apply it to study the molecular mechanism of signal recognition particle.

Signal Recognition Particle (SRP) and its receptor (SR) are GTPases responsible for targeting roughly one third of the proteome to the endoplasmic reticulum (ER) in eukaryotic cells, or to the bacterial plasma membrane. W e applied single molecule fluorescence microscopy to demonstrate that the SRP- SR GTPase complex, after initial assembly at the tetraloop end of SRP RNA, travels over 100 Å to the distal end of this RNA where rapi d GTP hydrolysis occurs. This movement is negatively regulated by the translating ribosome and, at a later stage, positively regulated by the SecYEG translocon, providing an elegant mechanism to ensure the productive exchange of the targeting and translocation machineries at the ribosome exit site with exquisite spatial and temporal accuracy. Our results show that large RNAs can act as molecular scaffolds that enable the facile exchange of distinct factors and precise timing of molecular events in a complex cellular process.

Seung-Jae Lee, Postdoctoral Researcher, Leemor Joshua-Tor Lab, Cold Spring Harbor Laboratory (HHMI)

Dr. Lee is visiting the Center in the Ha Group Lab from December 2011 to December 2012 to learn single-molecule TIRF microscopy and apply it to study the unwinding mechanism of E1 helicase from papillomavirus.

For more information, please visit http://joshua-torlab.cshl.edu/

Carlos Penedo, Lecturer in Biophysics, University of St Andrews

Dr. Penedo visited the Center from January 29 to February 8, 2012 to learn vesicle encapsulation methods of protein/DNA complexes, in particular those involving DNA repair helicases such as Hel308 and XPB/Bax1. The collaboration with the Center continues with students attending the CPLC Summer School and visiting the Center for additional training.

For more information, please visit http://www.st-andrews.ac.uk/~singlemol/singlemol.html

Ward Lopes, Assistant Professor of Physics, Williams College

Professor Lopes visited the Center in the Selvin Lab from July 17 to October 17, 2011. He worked on a way to get a holographic array to work in a microscope to allow multi-point (parallel) illumination. He contributed to two articles, both led by Ruobing Zhang, "Two-Photon 3D FIONA of Individual Quantum Dots in an Aqueous Environment", Nano Letters 11, 4074-4078 (2011), and a submitted article, "Multifocal Scan Microscopy Using an EMCCD for Single Molecule Imaging."

For more information, please visit http://web.williams.edu/wp-etc/physics/wlopes/

Elizabeth Komives, Professor of Chemistry and Biochemistry, UC San Diego

Professor Komives visited the Center from December 2009 to February 2010 to learn single molecule and temperature jump techniques and apply them to the IkappaB project. The collaboration is continuing with her postdoc visiting the Center regularly and several posters have been presented at various meetings.

For more information, please visit http://biomedsci.ucsd.edu/faculty/faculty_descrip.aspx?id=60