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Electrostatic Tweezers


Understanding how protein machinery of a biological cell packages, copies, and transcribes the encyclopedic information encoded in the genome requires the capability of applying forces like those in nature in a laboratory dish.Although optical traps, magnetic beads and an atomic force microscope can be used to apply forces to individual biomolecules, these tools employ tethers to transmit force from micron-size objects to the biomolecules of interest and generally have low throughput. The laboratories of Gregory Timp (ECE) and Aleksei Aksimentiev (Physics) at the University of Illinois, Urbana-Champaign have recently demonstrated a new tool for single molecule manipulation that obviates the use of tethers and offers high throughput ( In this method the gradient of the electrostatic potential is used to capture single DNA molecules and associated proteins in a solid-state nanopore. Once DNA is loaded into a nanopore, the electrostatic field is used to rupture the DNA-protein complex. Using a nanopore as electrostatic tweezers, the researchers have analyzed the forces binding a protein to a specific nucleotide sequence of a DNA molecule. Molecular dynamics simulations validated the electrostatic tweezers mechanism and provided accurate estimates of the forces involved.


Wired (San Francisco, Dec. 19) -- MC10 is a Wonka-like factory of crazy electronics concepts, from tiny skinborne “biostamps” to balloon catheters that can communicate back to doctors when they’re positioned correctly inside a clogged blood vessel. The company’s underlying technologies have been developed by U. of I. materials science and engineering professor John Rogers, who serves as the company’s chief technology officer.