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CPLC postdoctoral fellow Jingyi Fei publishes in Science

7/10/2015

Dr. Jingyi Fei published her article "Determination of in vivo target search kinetics of regulatory noncoding RNA" in Science.

Small noncoding RNAs (sRNAs) are important regulators for gene expression in all kingdoms of life. In the most common scenario, they function by a “search-and-execute” mechanism, i.e. base pairing with the target messenger RNAs (mRNAs) through a stretch of complementary sequence followed by translational repression and/or the induced-degradation of the mRNAs. CPLC postdoctoral fellow, Jingyi Fei, led a collaboration between the Ha group at CPLC and IGB and the Vanderpool group in the Department of Microbiology on campus, and  presented the first kinetic characterization of sRNA-mediated target mRNA degradation in vivo, using a super-resolution imaging and analysis platform. They used a sugar-phosphate stress induced sRNA, SgrS, as a model system, and found that the formation of SgrS-mRNA complex is the rate-limiting step, dictating its overall regulation efficiency. Surprisingly, binding of SgrS to the target mRNA is highly dynamic in vivo, with dissociate rate about two orders of magnitude larger than the in vitro measured, indicating an active recycling of sRNA from the sRNA-mRNA complex in the cell. Fine-tuning the target search kinetics allows additional control over regulation efficiency and contributes to setting the regulation priority among different target mRNAs by the same sRNA.

1/14/2014 Northwestern University

Research Experience for Undergraduates (REU) in Nanotechnology

Explore the new field of nanotechnology at Northwestern University (NU) - it's the best summer job you'll ever have!

As a participant in the 9-week NU-REU Program, you'll:

  • Work full-time on a nanotechnology research project
  • Have access to world-class equipment and facilities
  • Enjoy research seminars and field trips
  • Enhance your public speaking and technical writing skills
  • Participate in team events like the summer picnic, and explore Chicago

REU participants come from small and large schools, across the country.  They receive a stipend of $4,500 plus round-trip airfare and dormitory housing.

To be eligible for this program, you must be:

  • a U.S. citizen or permanent resident,
  • majoring in engineering or the sciences, and
  • have at least one year left to complete your undergraduate degree

The application deadline is February 17, and women and underrepresented minorities are strongly encouraged to apply.

For more information or to download an application visit: http://iinano.org/content/REU/index.htm or contact Margaret Connolly at (847) 467-4862 or email m-connolly@northwestern.edu.

This program is sponsored by the NSF and the NIH-NCI.

Margaret Connolly

Outreach Coordinator
International Institute for Nanotechnology 
Northwestern University

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